DIAGNOSTIC EFFICIACY OF IMMUNOCHROMATOGRAPHY TECHNIQUE FOR DETECTION OF VIRAL HEPATITISINFECTION IN SUSPECTED SERUM SPECIMENS
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Author:
NOSHEEN SHOUKAT
Citable URI :
https://vspace.vu.edu.pk/detail.aspx?id=385
Publisher :
Virtual University
Date Issued:
7/7/2020 12:00:00 AM
Abstract
A prospective study was conducted in order to evaluate the diagnostic efficacy of
immunochromatography assays for qualitative detection of hepatitis B surface antigen and
hepatitis C virus antibodies, using commercially available rapid test devices. Rural
population of district Chakwal visiting primary care hospitals for screening of hepatitis B and
hepatitis C virus infections was selected as study population. Coagulated blood specimens
were collected from a total of 500 individuals; serum was separated and stored at -20
C till
analysis. Necessary demographic information with previous exposure and screening history of
each individual was also recorded in pre designed performa. The stored serum specimens
were analyzed for detection of hepatitis B surface antigen and hepatitis C virus antibodies
through immunochromatography technique (ICT) and enzyme linked immunosorbant assay
(ELISA) using commercially available ICT rapid test devices and ELISA kits. Out of total
500 specimens analyzed through ICT for HBs Ag detection, 15 exhibited positive outcome
for the infection whereas analysis of the same specimens through standard ELIS test revealed
16 true positives. One of the ICT tested specimens was therefore recorded as false negative.
The sensitivity of rapid test device for HBs Ag detection was therefore 93.75% with 100%
specificity, 100% positive predictive value and 99.8% diagnostic accuracy. Similarly 34 of the
500 ICT tested specimens exhibited positive outcome for hepatitis C virus infection whereas
37 true positives were recorded when same specimens were analyzed through standard ELISA
test. The sensitivity of ICT rapid test device for HCV Ab detection was therefore 91.89% with
99.57% specificity, 94.44% positive predictive value, 99.35% negative predictive value and
99% diagnostic accuracy. The findings indicate that the rapid diagnostic immunoassays, in
spite of requiring minimum time and equipment, are not comparable with standard ELISA for
screening of hepatitis B and hepatitis C virus infections.
URI :
https://vspace.vu.edu.pk/details.aspx?id=385
Citation:
Shoukat,N(2019).DIAGNOSTIC EFFICIACY OF IMMUNOCHROMATOGRAPHY TECHNIQUE FOR DETECTION OF VIRAL HEPATITISINFECTION IN SUSPECTED SERUM SPECIMENS. Virtual University of Pakistan(Lahore, Pakistan)
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Final Version
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