Browse

The Virtual University, Pakistan’s first University based completely on modern Information and Communication Technologies, was established by the Government as a public sector, not-for-profit institution with a clear mission: to provide extremely affordable world class education to aspiring students all over the country.

Using free-to-air satellite television broadcasts and the Internet, the Virtual University allows students to follow its rigorous programs regardless of their physical locations. It thus aims at alleviating the lack of capacity in the existing universities while simultaneously tackling the acute shortage of qualified professors in the country. By identifying the top Professors of the country, regardless of their institutional affiliations, and requesting them to develop and deliver hand-crafted courses, the Virtual University aims at providing the very best courses to not only its own students but also to students of all other universities in the country.

  1. Home
  2. Thesis Detail

MOLECULAR CHARACTERIZATION OF LOCAL STRAINS OF Plasmodium vivax BASED ON PvCsp GENE

Download

Author: ZAINAB BIBI

Citable URI : https://vspace.vu.edu.pk/detail.aspx?id=382

Publisher : Virtual University

Date Issued: 7/7/2020 12:00:00 AM

Abstract

Plasmodium vivax is the most prevalent species and always remains a major cause of mortality and morbidity in endemic areas, accounting for at least 65 – 70% of all cases happening in Pakistan. However, Pakistan is deadly lacking information about the structure, role in drug resistance and population of P.vivax species. To get knowledge about the parasitic origin and diversity of P.vivax, the study of its population genetics is necessary. The PvCsp gene is considered as highly polymorphic in nature and much important for the survival of P.vivax under severe pressures of the environment in different areas. Also for the development of vaccine, this gene has been identified as a potential candidate. The present study focused at the investigation of the structure of the P. vivax populace through the diversity of PvCsp gene. To get the nucleotides sequences of the PvCsp gene, DNA extraction, PCR and Sanger sequencing of P.vivaxsamples collected from local areas of Rawalpindi and Islamabad.The sequences were aligned and analyzed by using Geneious prime and Bio Edit software. The phylogenetic trees were constructed using ML tree and neighbor joining tree methods. Many nucleotide differences, repeats, deletions were found at different places in the PvCsp.The study provided a lot of information about the variations or mutations in the PvCsp gene of our localP.vivaxstrains. It is concluded that local P. vivax strains of federal area of Pakistan depicted significant variations as compared to the Reference sequences. Therefore\, it is suggested that prevailing P. vivax strains of federal areas of Pakistan might be categorized as a different strain which need further investigations.


URI : https://vspace.vu.edu.pk/details.aspx?id=382

Citation: Bibi,Z(2019).MOLECULAR CHARACTERIZATION OF LOCAL STRAINS OF Plasmodium vivax BASED ON PvCsp GENE. Virtual University of Pakistan(Lahore, Pakistan)

Version : Final Version

Terms of Use :

Detailed Terms :

Journal :

Files in this item
Name Size Format
Fall 2019_MB720_MS170200474.pdf 4602kb pdf

Copyright 2016 © Virtual University of Pakistan