“STUDY OF GENETIC VARIANTSIN CRYAAGENE IN DIABETIC PATIENTSWITH CATARACT”
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Author:
RABIA RAFIQ
Citable URI :
https://vspace.vu.edu.pk/detail.aspx?id=380
Publisher :
Virtual University
Date Issued:
7/7/2020 12:00:00 AM
Abstract
Diabetes mellitus is a metabolic disease that brings intricacies in different organs of human.
Eye is one of the organs affected by diabetes mellitus. Cataract is one of the ocular
complications faced by the diabetic patient, regardless of the significant development in the
field of medical sciences. Cataract is increasing gradually but there is no proper treatment
except surgery. CRYAA gene plays a vital role in the maintenance and development of lens
due to the presence of alpha crystalline protein. CRYAA gene code for crystalline protein
which is comprises of 80-90 percent of soluble protein in lens that is exclusively required for
lens stability and transparency. CRYAA gene is located on chromosome 21 and encodes 173residue
protein.
We conducted this study to check mutation in the exon 1 and exon 2 of CRYAA gene in
diabetic cataract patients. Fifty patients were considered for this study. They were classified
into two groups. One group with patients of diabetic cataract, whereas the other group was
including diabetic patients without cataract. Blood of patients were collected from Razia eye
foundation hospital Jhelum after obtaining written consent. Blood was collected for DNA
extraction then Polymerase chain reaction was performed to amplify the exon 1 and exon 2 of
CRYAA gene as a preliminary step for sequencing by using Sanger sequencing method.
The sequencing analysis results indicate no mutation in the exon 1 and exon 2 of CRYAA
gene.
In the present study, we found that exon 1 and exon 2 of CRYAA gene are not associated with
diabetic cataract.
URI :
https://vspace.vu.edu.pk/details.aspx?id=380
Citation:
Rafiq,R(2019).STUDY OF GENETIC VARIANTSIN CRYAAGENE IN DIABETIC PATIENTSWITH CATARACT. Virtual University of Pakistan(Lahore, Pakistan)
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Final Version
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