SCREENING OF LACTOBACILLUS FERMENTUM (L.FERMENTUM) FROM BUFFALO MILK FOR THEIR POTENTIAL USE AS PROBIOTIC
Download
Author:
SANA ABID
Citable URI :
https://vspace.vu.edu.pk/detail.aspx?id=372
Publisher :
Virtual University
Date Issued:
7/6/2020 12:00:00 AM
Abstract
The demand of functional foods is on the rise and researchers are trying to develop nutritious
dairy products from well characterized bacterial strains. Lactobacillus is considered to be
important bacterial species, which have been recognized as probiotics for providing host
health through the improvement in the gut microbiota. The objective of present study was to
isolate and molecular characterize the Lactobacillus fermentum from Nilli Ravi buffalo milk
for its potential use as probiotic like fermented milk. Fifteen (15) L. fermentum were isolated
and studied for acid and bile salt tolerance. Out of fifteen strains, only 4 strains (NMCC-17,
NMCC-2, NMCC-28, and NMCC-14) were selected based on their maximum acid and bile
salt resistance. These strains were used for in vitro study for their probiotic potential based on
lysozymes tolerance, cholesterol assimilation, autoaggregation, hydrophobicity, lipolytic,
proteolytic, amylolytic, beta-galactosidase test, hemolytic activity and exopolysaccharide
production. NMCC-14 and NMCC-17 strains showed maximum probiotic potential. These
isolates were identified as L. fermentum (MK611941 and MK614164) by 16S rRNA gene
sequencing. Fermented milk sample inoculated with NMCC-14, and NMCC-17 showed
decreased in live cfu/g at 4
o
C at day 25. Bacterial count persistence is more than 7cfu /ml
which is good sign of health benefit requirement
URI :
https://vspace.vu.edu.pk/details.aspx?id=372
Citation:
ABID,S(2019),SCREENING OF LACTOBACILLUS FERMENTUM (L.FERMENTUM) FROM BUFFALO MILK FOR THEIR POTENTIAL USE AS PROBIOTIC,VIRTUAL UNIVERSITY OF PAKISTAN.(Lahore,Pakistan).
Version :
Final Version
Terms of Use :
Detailed Terms :
Journal :
Files in this item |
Name |
Size |
Format |
Fall 2019_ZOO720_ms160400102.pdf |
1616kb |
pdf |