GENETIC DIVERSITY AND PHLOGENETIC RELATIONSHIP OF ROHU FISH (LABEO ROHITA) FROM RIVER CHENAB AND ITS RESERVIORES BY USING SIMPLE SEQUENCE REPEAT METHOD
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Author:
HAFIZ MULAZIM HUSSAIN
Citable URI :
https://vspace.vu.edu.pk/detail.aspx?id=347
Publisher :
Virtual University
Date Issued:
7/4/2020 12:00:00 AM
Abstract
Labeo rohita (Rohu) is naturally occurring fish in the rivers of Pakistan, Bangladesh, India, Terai
region, Nepal, Burma, Sri-Lanka, Thailand, Vietnam and Laos etc. Despite its wide distribution
and status genetic data of Pakistani population is not available in the literature. Genetic
measurement of a species is not only significant for specific administration but also for
understanding its genetic variations. Various genetic and molecular methods are available for the
complete genetic analysis and phylogenetic relationships of species. This study involves simple
sequence repeat markers for the determination of genetic variation between various samples of
Labeo rohita specie, which were collected from Head Muhammad Wala, Muzaffargarh and
Khangarh regions of river Chenab, Pakistan. DNA was extracted from the muscles of fish by
(phenol:chloroform:isoamylalcohol) extraction method. PCR was performed for the
amplification of microsatellite regions. Through Polyacrylamide gel electrophoresis (PAGE),
scoring of bands for alleles was performed for the generation of genotypic data which was then
used for various indications of genetic diversity. Mean value of polymorphism for all markers
was 78.63%. Out of 543 loci detected 427 were found polymorphic in all samples. Allele number
fluctuated from 2 – 7 with average 3.58 values and allelic frequency was from 0.3333 to 1.0000
with average 0.6389. Gene diversity found was from 0.0000--0.7901 with average 0.5624 and
PIC value was 0.0994 -- 0.7618 with average. 0.4988. Mostly values of Nei’s genetic distance
among different populations of different areas of river Chenab were 0.6667, 0.7500, while values
of Jaccard’s similarity coefficient were 0.7500 and 0.9167.These values indicate that genetic
makeup of L. rohita individuals of different areas of river Chenab differ from each others. This
difference may be due to anthropogenic interventions, reproductive isolations and environmental
factors. Phylogenetic tree on the base of UPGMA divided all samples into three clusters. C1
xv
contains all samples of Khangarh region, C2 contains samples of Head Muhammad Wala and C3
contains all the samples of Muzaffargarh region. Cluster analysis shows that samples of one
region show similar genetic information but genetic differences are more with the fish of other
regions. It means that geographic isolation and different aquatic conditions have effect on
genetics of organisms.
URI :
https://vspace.vu.edu.pk/details.aspx?id=347
Citation:
Hussain, M(2019),GENETIC DIVERSITY AND PHLOGENETIC RELATIONSHIP OF ROHU FISH (LABEO ROHITA) FROM RIVER CHENAB AND ITS RESERVIORES BY USING SIMPLE SEQUENCE REPEAT METHOD,VIRTUAL UNIVERSITY OF PAKISTAN,(Lahore, Pakistan).
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Final Version
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