ISOLATION OF NEWCASTLE DISEASE VIRUSES FROM LIVE BIRDS MARKETS AND NATIONAL ZOOLOGICAL PARKS IN LAHORE DISTRICT
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Author:
MUHAMMAD AWAIS
Citable URI :
https://vspace.vu.edu.pk/detail.aspx?id=321
Publisher :
Virtual University
Date Issued:
7/4/2020 12:00:00 AM
Abstract
Avian orthoavulaviruse-1 (AOAV-1) including highly pathogenic strains, pose severe economic
and animal health concerns in developing countries. Live Bird Markets (LBMs) in Asian
countries are considered as hubs for the spread and maintenance of different avian infectious
diseases. In developing countries, poultry trade is characterized by complex chains comprising of
many factors with limited biosecurity. In Pakistan, there is lack of uniformity in practices used in
LBMs, leading to heavy impact in the spread of the disease. A cross-sectional survey was
conducted to identify risk factors associated with AOAV-1 infections and their genetic
characterization in LBMs from eight-towns of Lahore district during 2016-2018. A total of 189
poultry stalls (n=1015 birds) distributed in eight-towns were visited and a pool of 5-6
oropharyngeal swab samples was collected from each stall and tasted by RT-PCR for the Fusion
(F) gene. Ninety-one (91) of 189 swab samples were found positive for AOAV-1s.The overall
prevalence of the AOAV-1in poultry stalls was 48.14% (95% Cl = 41.13-55.24) observed. Data
for eleven potential risk factors acquired through detailed questionnaires were analyzed by
survey-weighted logistic regression and prevalence odds ratios (OR) for associated risk factors
were calculated. A total of 24 AOAV-1 isolates were sequenced to examine the circulating
genotype through complete F-gene. A phylogenetic analysis revealed that all isolates belonged to
sub-genotype VIIi, genotype VII class II. This study provides important information on
epidemiology and control of AOAV-Iin Pakistan and highlights the importance of implementing
surveillances and biosecurity practices in LBMs.
URI :
https://vspace.vu.edu.pk/details.aspx?id=321
Citation:
Awais , M(2019),ISOLATION OF NEWCASTLE DISEASE VIRUSES FROM LIVE BIRDS MARKETS AND NATIONAL ZOOLOGICAL PARKS IN LAHORE DISTRICT,VIRTUAL UNIVERSITY OF PAKISTAN.(Lahore,Pakistan).
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Final Version
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