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CHITOSAN ENCAPSULATEDPOLYGALACTURONASE ITS KINETIC CHARACTERISATION FOR INDUSTRIAL EXPOLITATION

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Author: ZUNEIRA SADDIQUE


Citable URI : https://vspace.vu.edu.pk/detail.aspx?id=314

Publisher : Virtual University

Date Issued: 7/4/2020 12:00:00 AM


Abstract

Polygalacturonase (PG) is a group of pectinases that possess hydrolytic functions and are capable to cleave the a-1, 4 glycosidic bonds between the two non-esterified galacturonic acid residues in pectin and pectic substances. These enzymes are used commercially, such as paper, textile, cellulose, and food industries and have many important industrial applications. It is the most important tool for the purification of galacturonic acid and degradation of pollutants. In the present study substrates orange peel and sugar cane bagasse were used for the production of galacturonic enzymes by Trichoderma hazrianum through Solid State Fermentation (SSF). The highest yield of enzymes produced was 183.0U/mL. This highest yield was obtained at pH7 temperature 35°C, inoculums size 5mL, moisture level 70%, and days of incubation5 nitrogen level 2g.The purified enzyme has been further selected for ammonium sulphate precipitation and gel filtration chromatography. The purified enzyme has been characterized through enzymatic kinetic for study of its characterization temperature 45°C, pH8 and Km0.25, Vmax0.05 values. The immobilization was observed through chitosan. The immobilized enzyme showed the highest activity at temperature 45°C and pH6. For industrial application its activity was observed to decrease turbidity and increase fruit juice clarification.


URI : https://vspace.vu.edu.pk/details.aspx?id=314

Citation: Siddique,Z(2019).CHITOSAN ENCAPSULATEDPOLYGALACTURONASE ITS KINETIC CHARACTERISATION FOR INDUSTRIAL EXPOLITATION. Virtual University of Pakistan (Lahore, Pakistan)

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