ASSOCIATION STUDY OF SINGLE NUCLEOTIDE POLYMORPHISMIN THE UPSTREAM REGION OF TUMOR NECROSIS FACTORALPHA GENE WITH ASTHMA
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Author:
MUHAMMAD ABDULLAH
Citable URI :
https://vspace.vu.edu.pk/detail.aspx?id=312
Publisher :
Virtual University
Date Issued:
7/4/2020 12:00:00 AM
Abstract
Asthma is an obstructive airway inflammatory disease which causes narrowing of airway,
airflow obstruction and produces wheezing sounds. Tumor necrosis factor a
polymorphism leads to inflammation and provoke asthma which lead to morbidity and
mortality in the population. The objective of the study was to look into single nucleotide
polymorphism in upstream region of TNF a at -238 and -308 by ARMS PCR technique in
asthmatic and control group. It was a prospective study constituting 17 clinical diagnosed
asthmatic patients (Diseased) and 19 non-asthmatic patients (Control). The female was 6
(31.6%) and male were 13 (68.4%) in control group while female were 9 (52.9%) and
male were 8 (47.1%) in asthmatic group. Mean age (49.59±15.82 year) and BMI
(21.96±06.67 kg/m2) were recorded for asthmatic group while mean age and BMI for
control group were recorded to be 32.82±12.50 year and 18.87±05.17kg/m
, respectively.
PCR amplification of TNF a gene was done in a thermocycler for -238 and -308
genotype analysis by the ARMS technique and statistical analysis showed that there was
association between asthma and TNF a polymorphism at -238 G>A (p < 0.0001) and
-308 G>A (p < 0.0086). Clinical genetic testing should be recommended to use TNF a
polymorphism as a biomarker in asthmatic patients to reduce its adverse effects.
URI :
https://vspace.vu.edu.pk/details.aspx?id=312
Citation:
Abdullah,M(2019).ASSOCIATION STUDY OF SINGLE NUCLEOTIDE POLYMORPHISMIN THE UPSTREAM REGION OF TUMOR NECROSIS FACTORALPHA GENE WITH ASTHMA. Virtual University of Pakistan (Lahore, Pakistan)
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