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Isolation and Identification of polyhydroxy alkanoates accumulating bacteria from sugar-cane industry waste

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Author: FARZANA YASMIN

Citable URI : https://vspace.vu.edu.pk/detail.aspx?id=299

Publisher : Virtual University

Date Issued: 1/15/2020 12:00:00 AM

Abstract

Polyhydroalkanoates (PHAs) are polyesters that are synthesized by different microorganism and stored as granules in microbes. They are used as a food under starvation. Studies have shown that physical properties of different PHAs resemble significantly to the petroleum based plastics and polyethylene. Additionally, microbial PHAs can be spun, mould and converted into filaments. On the other hand, PHAs are biodegradable material. Such promising features make PHAs as a potential candidate for next generation plastic as it will reduce the pollution caused by the petroleum based plastics. However, PHAs are costly as compared to the different petroleum based plastics. Therefore, researchers are trying to identify new microbes that may produce high amount of PHAs from waste carbon sources. They can accumulate high concentration of PHAs. During the crushing season sugar industries are producing tones of organic waste, specifically they are draining a considerable amount of sugar in canals. This can be a source of food for different types of bacteria, specifically for PHA accumulating bacteria. In this study, different PHAs accumulating bacteria were identified from the swage of sugar industry waste. Five soil and sewage samples were collected and stored at room temperature. For isolation and identification of PHAs accumulating bacteria, Samples were allowed to grow on LB agar medium plates supplemented with 1% Nile blue A. Different concentrations of Nile blue A were used for different incubation periods and colonies were screened. All these colonies were observed under ultraviolet light; PHAs accumulating bacteria were showed bright orange color inflorescence while non PHAs accumulating bacteria appeared white. The PHAs accumulating bacteria were selected and then amplified for 16S rRNA gene sequencing through PCR. In this study we have identified five PHAs accumulating bacteria from the waste of sugarcane industry by using the chemical and sequence analysis. These bacteria can be used for the laboratory and large scale production of different types of PHAs.


URI : https://vspace.vu.edu.pk/details.aspx?id=299

Citation: Yasmin, F(2019). Isolation and Identification of polyhydroxy alkanoates accumulating bacteria from sugar-cane industry waste. Virtual University of Pakistan.(Lahore, Pakistan).

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