Browse

The Virtual University, Pakistan’s first University based completely on modern Information and Communication Technologies, was established by the Government as a public sector, not-for-profit institution with a clear mission: to provide extremely affordable world class education to aspiring students all over the country.

Using free-to-air satellite television broadcasts and the Internet, the Virtual University allows students to follow its rigorous programs regardless of their physical locations. It thus aims at alleviating the lack of capacity in the existing universities while simultaneously tackling the acute shortage of qualified professors in the country. By identifying the top Professors of the country, regardless of their institutional affiliations, and requesting them to develop and deliver hand-crafted courses, the Virtual University aims at providing the very best courses to not only its own students but also to students of all other universities in the country.

POLYMORPHIC VARIATION AND PHYLOGENY OF THE MYOSTATIN GENE EXON 1 IN WILD PUNJAB URIAL (Ovis vignei punjabiensis)

Download

Author: ASAD ULLAH GHALIB


Citable URI : https://vspace.vu.edu.pk/detail.aspx?id=119

Publisher : Virtual University

Date Issued: 4/23/2018 12:00:00 AM


Abstract

Myostatin encoded by MSTN gene is very important growth factor related to the ß super family. Its expression causes the fat production and muscle deregulation. The variation in MSTN gene has been associated with distinction in muscularity in animals. Double muscling can be observed in several farm animals including goat, sheep, pig, cattle, horse, and also in fish. The knowledge of variation in MSTN gene is necessary to be explored in wild animals. In the current study we hypothesized that the variation within the MSTN gene may be important in identifying the genomic region that would be responsible for significant phenotypes. Therefore the MSTN gene previously referred as GDF8 gene was studied in the Punjab Urial, considered it as one of the wild ancestors of domestic sheep found in the North of the Punjab province of Pakistan. The samples from wild and captivity were subjected to DNA extraction, PCR and sequencing. The sequenced product (373bp) includes 5’ untranslated region (UTR), 67 base pair upstream from the start codon (ATG), complete exon 1 of the MSTN gene and partial intron 1 region. The coding region contained 372 bp. In untranslated region one substitution and one indel (deletion) was observed. One substitution (A>C) was reported on position 3564 of the MSTN gene and the indel (deletion) was identified at 3574 bp position. All the sequences were compared with the reported sequences on GenBank. The analysis shows the presence of thiamine in the wild sequence .This is the preliminary study of MSTN gene in the wild Punjab Urial. Further studies might give us a deep insight of important MSTN gene in wild as well as domestic animals.


URI : https://vspace.vu.edu.pk/details.aspx?id=119

Citation: Ghalib, A.U,(2017). POLYMORPHIC VARIATION AND PHYLOGENY OF THE MYOSTATIN GENE EXON 1 IN WILD PUNJAB URIAL (Ovis vignei punjabiensis). Virtual University of Pakistan (Lahore. Pakistan).

Version : Final Version

Terms of Use :

Detailed Terms :

Journal :

Files in this item

Name Size Format
Fall 2017_BT720_ms150400976.pdf 906kb pdf


Copyright 2016 © Virtual University of Pakistan